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제목 Gene Editng Detection of CRISPR/Cas 9 from S.aureus. (ELISA based Measurement Assay Kit) 출시!
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작성일 2017-08-17 09:18:41
New, smaller S. aureus based CRISPR/Cas9 ELISA Kit for precision genome editing studies
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15% Off New CRISPR/S. aureus Cas9 ELISA*

 
See our latest ELISA kit, the new EpiQuik™ CRISPR/SaCas9 (S. aureus) Assay ELISA Kit (Colorimetric) designed to specifically measure CRISPR-associated protein 9 (Cas9 and dCas9) amounts from S. aureus - a smaller Cas9 ortholog that's best suited for viral vector applications. Quantification can be done quickly and easily using purified SaCas9 nuclease or whole cell extracts isolated from tissues and cultured cells of various species.
  • Quick and easy 3 hour procedure
  • Innovative colorimetric assay to quantitatively measure Cas9 protein amount without the need for electrophoresis
  • Great for screening Cas9-transfected clones
  • Detection limit as low as 0.1 ng/well 
  • Flexible strip microplate format for manual or high throughput analysis
*15% off limited time offer. No code needed. Ends 11/30/17.

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 SaCas9 proteins are spiked into 1 µg of K562 cell extracts at the different concentrations. The amount of the SaCas9 proteins was measured using the EpiQuik™ CRISPR/SaCas9 (S. aureus) Assay ELISA Kit (Colorimetric).

For Antibody-Based Gene Editing Studies
The CRISPR/Cas9 system has proven to be an invaluable tool for targeting and modifying a genetic sequence in gene editing and genome engineering applications. EpiGentek's high quality CRISPR/Cas9 Monoclonal Antibody [7A9] can be used for: 
  • Detection of CRISPR/Cas9 expression in target cells by WB, IF, IP or ELISA to confirm whether gRNA and Cas9 vectors are successfully transfected
  • Detection of genome-wide off- and on-target effects of CRISPR/Cas9 on the specific gene sites by Cas9 ChIP-seq in CRISPR genome editing
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Featured Open Access Article
Article: Short-term sleep deprivation leads to decreased systemic redox metabolites and altered epigenetic status. By Trivedi MS et. al.

Researchers demonstrated that overnight total sleep deprivation (SD) results in redox-based global DNA methylation changes in the plasma of young adult humans. In particular, they found that plasma levels of the antioxidant glutathione (GSH) became reduced with a related elevation in oxidative damage. They also observed lower levels of redox metabolite ATP, thus signifying a modification in mitochondrial functioning. In the study, DNA isolation from plasma samples was done using our FitAmp Plasma/Serum DNA Isolation Kit, and global DNA methylation levels were measured using our MethylFlash Methylated DNA 5-mC Quantification Kit.

Read more [via PLoS One]
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