Global DNA Methylation in Just One-Step and 2 Hours

Measuring Global 5-mC just got better with our new MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric). This kit allows you to quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, "one-step" ELISA-like format. 

• Fast - Reduced steps allow you to finish in only 2 hours
• Robust - Improved kit has a greater "signal window" with reduced variation between replicates
• Convenient - Inherently low background noise without the need for DNA denaturation and plate blocking steps
• Sensitive - Detection limit can be as low as 0.05% methylated DNA from 100 ng of input DNA 
• Specific - No cross-reactivity to unmethylated or hydroxymethylated cytosine 
• Universal - Included positive and negative controls allow for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA  
• Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis
• Flexible - Strip-well microplate format for manual or high throughput analysis

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** MethylFlash™ Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit available soon!  **

Schematic procedure for the MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit.

Accurate quantification of 5-mC content of various DNA samples from different species using the MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit. The results are closely correlated with those obtained by HPLC-MS.