Description
The expression and solubility of recombinant proteins can be increased by tagging with Glutathione S-transferase (GST). GST-tag binds to glutathione with high affinity and specificity enabling purification of GST-tagged proteins using glutathione-based affinity resins. BioVision’s EZCatch™ GST-Spin Purification Kit is designed for fast, specific and easy purification of such GST-tagged proteins from cell lysates. This kit contains 10 easy-to-use spin columns allowing one-step high quality affinity purification of different GST-tagged proteins. In addition, the kit contains a proprietary Lysis Buffer allowing quick preparation of clear lysates from cell pellets without the need for sonication. Eluted proteins can be directly used for downstream analysis.
Product details
| Cat # +Size | K861-10 |
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| Size | 10 columns |
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| Highlights | Binding Capacity: Each Spin column can bind upto 250 µg of recombinant protein from 1-5 ml of cell culture or up to 0.25 g of cell pellet.
Column Content: Each Spin column contains 100 µl of high performance Glutathione Sepharose beads (Cat # 6555).
Bead Content: A 50% slurry (100 µl of settled resin is equivalent to 200 µl of 50% slurry) in 20% ethanol.
• Rapid purification of GST-fusion proteins from multiple samples
• Optimization of growth conditions for the expression of recombinant proteins
• Pull-down of purified GST-fusion proteins for subsequent Western blot (with GST Antibody – Cat # 3997), ELISA and other downstream applications |
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| Storage Conditions | 4°C |
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| Shipping Conditions | Gel Pack |
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| USAGE | For Research Use Only! Not For Use in Humans. |
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