Product Info

Stellaris® FISH Probes, Canine POLR2A with Quasar® 570 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect POLR2A transcripts in specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against polymerase (RNA) II (DNA directed) polypeptide A POLR2A a.k.a. (NCBI gene ID:479483) and nucleotides 479-3050 of XM_852751.3 . The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.

Technical specs

▶ Properties:

• Absorption Maximum (Lambda Max): 

  548

• Fluorescence Maximum: 

  566

▶ Product usage:

Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.

▶ Storage and handling:

• Shipping conditions: 

  Ambient

• Storage conditions: 

  +2 to +8 °C