BioVision’s Human BMP-5 (Bone morphogenetic proteins-5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-5. This assay employs an antibody specific for human BMP-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-5 is typically less than 0.25 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.